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UNIVERSITY OF SOUTHERN DENMARK
O- GlcNAcylation promotes osteoclastogenesis and dictates the resorption mode by osteoclasts
Ph.D : Thaise Mayumi Taira Supervisor: Prof. Dr. Sandra Yasuyo Fukada
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UNIVERSITY OF SÃO PAULO SCHOOL OF PHARMACEUTICAL SCIENCES OF RIBEIRÃO PRETO
Trap MMP-9 CtsK Boyle et al., 2003 Kalervo-Vaananen et al., 2008 Qin et al., 2012 Teti, 2013
Glucose HEXOSAMINE BIOSYNTHETIC PATHWAY (HBP)
ATP
ATP
ATP
ATP
ATP
ATP
What is the role of O- GlcNAcylation on osteoclastogenesis ?
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\OCfusion 02-TMT-19 O-GlcNAc pictures\10B_Control 10x.tif
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\OCfusion 02-TMT-19 O-GlcNAc pictures\6C_0.03mM O-GlcNAc 10x.tif
Control
GlcNAc (0.3 mM )
O- GlcNAcylation participates on osteoclasts differentiation and fusion
* Paper under review
A
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\OCfusion 02-TMT-19 O-GlcNAc pictures\10B_Control 10x.tif
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\OCfusion 02-TMT-19 O-GlcNAc pictures\6C_0.03mM O-GlcNAc 10x.tif
Control
GlcNAc (0.3 mM )
Control
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\Ofusion 02-TMT-19 OSMI-1 pictures\3E Control 10x.tif
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCfusion-02-TMT-19-OSMI-1, O-GlcNAc TRACP activity\Ofusion 02-TMT-19 OSMI-1 pictures\9D 30nM OSMI-1 10x.tif
OSMI-1 (30 nM )
O- GlcNAcylation participates on osteoclasts differentiation and fusion
* Paper under review
A
B
(OGT inhibitor )
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCreesorption 02-TMT-19 pictures of bone slices\OCresorption 02-TMT-19 O-GlcNAc\A4 0.03mM O-GlcNAc 20x.tif
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCreesorption 02-TMT-19 pictures of bone slices\OCresorption 02-TMT-19 O-GlcNAc\B8 Control 20x.tif
O- GlcNAcylation affects the type of osteoclasts resorption
Control
GlcNAc (0.3 mM )
A
* Paper under review
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCreesorption 02-TMT-19 pictures of bone slices\OCresorption 02-TMT-19 O-GlcNAc\A4 0.03mM O-GlcNAc 20x.tif
L:\PATO\Klinisk Cellebiologi\Thaise\Results\OCVideo 02-TMT-19 OSMI-1 and O-GlcNAc differentiation resorption\OCreesorption 02-TMT-19 pictures of bone slices\OCresorption 02-TMT-19 O-GlcNAc\B8 Control 20x.tif
O- GlcNAcylation affects the type of osteoclasts resorption
Control
GlcNAc (0.3 mM )
Control
OSMI-1 (30 nM )
A
B
* Paper under review
(OGT inhibitor )
Control
GlcNAc 0.03 mM
Bone and Osteoclasts
O- GlcNAcylation affects the type of osteoclasts resorption
Control
GlcNAc 0.03 mM
Bone and Osteoclasts
O- GlcNAcylation affects the type of osteoclasts resorption
Control
OSMI-1 1 n M
O- GlcNAcylation affects the type of osteoclasts resorption
Bone and Osteoclasts
Control
OSMI-1 1 n M
O- GlcNAcylation affects the type of osteoclasts resorption
Bone and Osteoclasts
✓ O- GlcNAcylation stimulates osteoclastogenesis in vitro; ✓ O- GlcNAcylation coordinates osteoclasts’s agressiveness for bone resorption .
CONCLUSION
Acknowledgment
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Profa. Sandra Y . Fukada – Supervisor (FCFRP-USP) Prof. Kent Søe - Colaborador (SDU – Denmark ) Prof. Erivan Shineider Ramos Junior – Colaborator (Augusta University ) Cecília Costa do Carmo e Silva – Student (FCFRP-USP) Mayara Santos Gomes – Laboratory Technitian (FCFRP-USP) Jacob Bastholm Olesen – Laboratory Technitian (SDU)
Thank you for your attention !
t haise.taira@usp.br
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oop | Erivan Schnaider Ramos-Junior