Validation of red deer's (Cervus elaphus) oxygen isotope composition in enamel carbonate (δ18Oec) to reconstruct paleotemperatures and seasonality: evidence from known specimens from the Iberian peninsula

Validation of red deer’s ( Cervus elaphus ) oxygen isotope composition in enamel carbonate (δ 18 O ec ) to reconstruct paleotemperatures and seasonality: evidence from known specimens from the Iberian peninsula.

Background. aldures p.reptnas (091/081) / - (091/0801 = (0%11!) oste.

(Mid to high latitudes) Temperature Relative humidity Seasonality Source effects Path effects In-cloud, sub-cloud processes Amount of precipitation 5180 precipitation Mix from different time/site Source of water Evaporation Seasonality 5180 drinking water Temperature Relative humidity Physiology Water vapour C02 Excreted water ö180 body water Metabolic water Atmospheric O Structural 02 in food Water in plants Diagenesis (fossils) Amelogenesis 5180 carbonate.

After Jouzel et al. 1994. After Dansgaard et al. 1994.

d18O (‰) -5.8780000000000001 -5.3090909090909086 -4.9800000000000004 -5.2372727272727273 -4.2134999999999989 -4.1470000000000002 -3.5322222222222219 -2.7110000000000003 -4.7739999999999991 -5.1027272727272726 -5.9079999999999995 -5.5418181818181811 Tª (ºC) 10.963636363636363 11.227272727272727 12.67272727272727 13.336363636363636 15.290909090909093 18.154545454545453 19.354545454545452 19.981818181818181 18.954545454545457 16.518181818181816 13.145454545454545 11.345454545454546.

ooz J aweo xodé ooz 0 uomsod 8. . After Tejedor-Rodríguez et al. 2021.

abstract. Why red deer ? Extant species with a wide geographic /temporal distribution Homeotherm and sedentary D’Angela and Longinelli 1990, Fricke et al. 1998, Stevens et al. 2011 provide a good expectations for its applicability.

. . Material and methods. . M 3 N=7 4♂, 3♀. Hypoconid 8 to10 samples per tooth 2mm spaced.

abstract. N=6. abstract. N=1. Santander SCgbSQÜ1.

Relate climate information of the specific year (δ 18 O mw , temp.) that red deer’s teeth are integrating (δ 18 O ec ): δ 18 O mw : GNIP (GNIP, IAEA/WMO, 2015) closest stations and OIPC (Bowen 2017) Temperature (AEMET stations at the sampled area).

Cementochronoly to calculate age. E-vu. 22-P50i.

Transfer functions applied VPDB VSMOW : δ 18 O ec (VSMOW) = 1.03092 [ δ 18 O ec (VPDB) ] + 30.92 Coplen et al., 1983 (NBS 19 by Coplen , 1996 ) δ 18 O ec  δ 18 O dw : δ 18 O dw = [δ 18 O ec - 33.63] / 0.998 ( Iacumin et al.,1996) δ 18 O dw (≈ δ 18 O mw ) temp. ( Rozanski et al., 1992): Temperature (°C) = [δ18Odw + 14.35] / 0.59 Maximum uncertainties in the δ 18 O values: 1.3‰ for Eq.2 and 2.2°C for Eq.3..

Results and discussion. Locality δ 18 O ec (‰ vs. VPDB*) Max Min Mean Median Range Riaño (N=6) -4,7 -9,3 -7,2 -7,3 4,6 Hosquillo -1,6 -4,1 -3,1 -3,1 2,4.

— 11 Dist..

Riano, sp Crown. height Rum Island, I-JK 8 Crcmn heig ht North Croatia Crown. height 111 — RA-13 — Ä"ty85 ¯ 85 Surtæy 94 Grand Teton, WY 821 Hosqui110, sp 8 28 27 S Killarney, IR o Crown _ he ight Crcwn _ ght Crown. height — NMINH 200141.1.

Riaöo, SP 26 9 24 20 Label RA-13 15 10 Dist. from ERJ.

ooo -1.00 -2.00 J _3.00 -4 00 RAI, RA13 (this study) WY-2 (Fricke et al. 1998) Stevensetal. 2011 Brown and Chapman, 1991.

OIPC δ 18 O mw (‰) vs. inferred δ 18 O dw (‰, Eq. 2) differences GNIP δ 18 O mw (‰) vs. inferred δ 18 O dw (‰, Eq. 2) differences Max Min Range Mean Max Min Range Mean RA-1 2.8 1.4 0.7 2.0 3.4 2.1 0.6 2.2 RA-13 3.4 0.0 1.5 0.8 1.0 -1.3 0.4 -0.9 RA-3 -0.2 2.3 1.0 2.9 RA-8 1.6 3.3 2.8 4.0 RA-307 2.8 3.1 RA-111 -0.8 1.5 1.9 3.4 Hos N22 -4.7 -1.9 -5.5 -1.8 RMSD 3.1 2.1 1.2 2.2 2.5 2.8 0.5 2.8.

Estimated temperature (⁰C, Eq. 3) vs. measured (⁰C, AEMET) differences Max Min Range Mean RA-1 -4.9 0.5 -5.5 3.1 RA-13 -6.4 3.2 -9.6 5.3 RA-3 0.2 1.7 RA-8 -0.8 0.2 RA-307 2.0 RA- 111 4.6 3.6 Hos N22 16.2 9.0 RMSD 5.7 7 7.8 4.5.

Estimated temperature (⁰C, Eq. 3) vs. measured (⁰C, AEMET) differences Max Min Range Mean RNCR population -5.8 -0.8 -5.0 2.5.

Conclusions. Enamel δ 18 O carbonate values ( δ 18 O ec ) of sequential sub- samples from red deer third molars show a pattern related to that observed in meteoric waters. This pattern appears highly variable even in individuals born in the same year. Two of the samples show a clear maximum (summer) and minimum (winter) δ 18 O ec value that allowed reconstructing an estimated nine month period for mineralization of the M3 enamel..

The correspondence between individual maximums, minimums, range, and mean δ 18 O ec values and year-specific climatic data is poor, although the values for the whole of the population appear to be approximate. Red deer δ 18 O ec from third molars of multiple individuals may be a useful proxy to recover information about past climate temperature and seasonality. Modern analog analyses are very useful to understanding ancient ecosystems, particularly for species that are still extant..

You just want me for my teeth. Thank you for your attention !.