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Crab?

Crab?

Crab?

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Mission Impossible? Or Possible?

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FROM DINNER TO BIOMEDICAL APPLICATIONS: THE VERSATILITY OF CRABS

PRESENTER : IMRANAH SAID MOHAMED STUDENT ID : 16005560 PROGRAMME : BACHELOR OF ELECTRICAL & ELECTRONICS ENGINEERING

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FOCUS OF THE LECTURE

PROPERTIES OF CHITIN & ITS DERIVATIVES

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MODIFICATION OF CHITOSAN

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CHITOSAN APPLICATION IN WOUND MANAGEMENT

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Keratinocytes Growth factors & cytokines Collagen Elastic fibers Fibroblasts Hair follicle Blood vessels Blood vessel •t'fibrin Clot PDGFAB% Wound Site

SKIN & WOUND COMPOSITION

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4 STAGES OF WOUND HEALING 2. Innammatay 3. ProllÉrattve 4. Remodeliræ

4 STAGES OF WOUND HEALING

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CHALLENGES/COMPLICATIONS: SCARRING LOSS OF HAIR FOLLICLES LOSS OF SWEAT GLANDS

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WHAT IS CHITIN?

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HARD SHINY OUTER CASING OF ARTHROPODS

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RUDN university Chitin CH20H OH NHR CH20H OH NHR Antibacterial activity Catalytic activity HO Chitin HO derivatives NH

ANTI-BACTERIAL BIODEGRADABLE & NON-TOXIC

CHITIN IS A FIBROUS SUBSTANCE CONSIST OF NATURAL POLYMER

CHITIN DERIVATIVES

CHITIN

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Crustaceans Shell Wash and crush Deproteinate (NaOH) Chitin Demineralise (HCI) Decolourise

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CHITIN IS INSOLUBLE IN MOST ORGANIC SOLVENTS!!!

PROBLEM

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CHITIN

CHITOSAN

CHITIN- DEACYTYLASE

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PROS & CONS OF CHITOSAN

PROS CONS ANTIFUNGAL POOR MECHANICAL PROPERTIES BIODEGRADABLE BIOCOMPATIBLE

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HERBS & SPICES ENHANCE TASTE

ADDITIVES ENHANCE MECHANICAL STRENGTH

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Forms ba agai Pm•ides protein healing

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day -7 day O day 4 Dropping the sample day 8 Harvest Habituation Circular excision wound model

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RESULTS & DISCUSSION

NON-TREATED

WATER

CHITIN NANOFIBRIL

CHITOSAN NANOFIBRIL

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CHITIN & CHITOSAN

ANTIMICROBIAL

ANTIFUNGAL

NON-TOXIC

BIOCOMPATIBLE

ANTIBACTERIAL

BIODEGRADABLE

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CHITIN & CHITOSAN

WOUND MANAGEMENT

BIOSENSORS DRUG DELIVERY TISSUE ENGINEERING ANTICANCER EFFECTS

WHY STOP HERE?