Name: PptxGenJS Presentation
Uploaded: 2024-09-13
Duration: 5 min 45 s
Description: Unveiling Osmosis: A Biotech Experiment. Welcome to an exciting journey into the world of osmosis! This experiment delves into the fascinating process of how water moves across semi-permeable membranes, driven by differences in concentration. We'll be using eggs, salt, and water to explore this fundamental concept in biology..

Unveiling Osmosis: A Biotech Experiment. Welcome to an exciting journey into the world of osmosis! This experiment delves into the fascinating process of how water moves across semi-permeable membranes, driven by differences in concentration. We'll be using eggs, salt, and water to explore this fundamental concept in biology.. 

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Welcome to an exciting journey into the world of osmosis! This experiment delves into the fascinating process of how water moves across semi-permeable membranes, driven by differences in concentration. We'll be using eggs, salt, and water to explore this fundamental concept in biology.

A person in a lab with a plant in a petri dish

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Contribution: Created this PowerPoint, and the Leader of the Group.

Shawn Matthew Flores. A child making a face Description automatically generated. 

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Experiment Materials: Eggs, Salt, and Water. 1. Eggs. 

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Experiment Materials: Eggs, Salt, and Water

Our experimental subjects! These eggs will serve as our semi-permeable membranes. They're made of a tough outer shell and a thin, flexible inner membrane called the vitelline membrane. This inner membrane acts as a semi-permeable barrier, allowing some substances to pass through while blocking others.

The key ingredient in our osmotic solutions! We'll be using salt in various concentrations to create different environments that affect water movement in the eggs. The salt ions in solution will play a crucial role in driving the osmotic process.

The solvent in our experiment! Water is essential for osmosis, acting as the medium for the movement of substances across the egg's membranes. We'll be using distilled water for this experiment to ensure consistent results.

Experimental Conditions: No Salt, Pinch of Salt, Tablespoon of Salt. 

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Experimental Conditions: No Salt, Pinch of Salt, Tablespoon of Salt

This condition serves as our control group. The eggs in this group will be placed in a solution of pure distilled water. This will allow us to observe the normal behavior of the egg in a neutral environment.

This condition represents a slightly hypertonic solution, where the salt concentration is slightly higher outside the egg than inside. We expect to see a subtle change in the egg's size and appearance as water moves out of the egg to balance the concentration difference.

This condition represents a significantly hypertonic solution, where the salt concentration is much higher outside the egg than inside. We anticipate a more pronounced change in the egg's size and appearance as water moves out of the egg in an attempt to achieve equilibrium.

S!SOLUSO. Hypothesis: Predicted Outcomes of Osmosis. 

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Hypothesis: Predicted Outcomes of Osmosis

We predict that the egg in this condition will not experience any significant change in size or appearance. This is because the water concentration inside and outside the egg is the same, so there is no net movement of water across the membrane.

We expect the egg to shrink slightly in this condition due to water moving out of the egg into the surrounding solution. This occurs because the salt concentration is higher outside the egg, causing water to move from an area of higher concentration (inside the egg) to an area of lower concentration (outside the egg).

In this condition, we anticipate a more noticeable shrinkage of the egg due to the significant concentration difference. The high salt concentration outside the egg will create a stronger osmotic gradient, causing a larger volume of water to move out of the egg, leading to a more pronounced reduction in its size.

Procedure: Step-by-Step Experiment Setup. Step 1: Prepare the Eggs. 

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Carefully remove the shell from the eggs. This can be done by gently rolling the egg on a hard surface to crack the shell, then peeling it away with your fingers. Be sure to keep the inner membrane intact. It's essential for our experiment because this membrane acts as a semi-permeable barrier for water movement.

Create three separate solutions: one with pure distilled water, one with a pinch of salt, and one with a tablespoon of salt. Measure out 50 mL of distilled water for each solution. For the salt solutions, add the specified amount of salt to the water and stir well to ensure it dissolves completely.

Carefully place one shelled egg into each of the prepared solutions. Make sure the eggs are fully submerged in the liquid. Leave the eggs in their respective solutions for at least 24 hours to allow sufficient time for osmosis to occur.

After 24 hours, carefully remove the eggs from the solutions. Observe the changes in their size and appearance. Note any differences in size and shape between the eggs in the three different solutions. Record your observations in a data table or notebook for later analysis.

Observations: Changes in Egg Size and Appearance. 

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Observations: Changes in Egg Size and Appearance

Observe the egg's shape, texture, and color.

00 too too too 100 Uett,nntoc ) 2100 2000. Data Analysis: Comparing Experimental Conditions. 

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Data Analysis: Comparing Experimental Conditions

Gather the data you collected from your observations. Record the initial and final circumferences of the eggs in each condition and any significant changes in their appearance. It's helpful to organize this data in a table or spreadsheet for easy analysis.

Create a visual representation of your data. A bar graph is a suitable way to show the differences in egg size for each experimental condition. This will help you easily compare the results and identify any trends.

Analyze the data to understand the relationship between salt concentration and the movement of water across the egg membrane. The visual representation of your data can help you draw conclusions about the effectiveness of osmosis in each condition.

Conclusion: Understanding Osmosis through Experimentation. 

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Conclusion: Understanding Osmosis through Experimentation

Compare your observations and data analysis with your initial hypothesis. Did your experiment support your predictions about the effect of salt concentration on osmosis in the egg? Did the results align with your expectations?

The experiment demonstrates how osmosis works. In hypertonic solutions, where the solute concentration is higher outside the egg, water moves out of the egg to try to balance the concentration difference. This causes the egg to shrink. In hypotonic solutions, where the solute concentration is lower outside the egg, water moves into the egg, causing it to swell.

This experiment provides a foundation for further exploring osmosis and other cell membrane processes. You could experiment with different concentrations of salt, other types of solutes, or different types of membranes to further investigate the intricacies of osmosis.

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