Title: Synergistic Trichogenic Efficacy of Wedelia chinensis and Cassytha filiformis Emulgel in Murine Models

[Virtual Presenter] Title: Synergistic Trichogenic Efficacy of Wedelia chinensis and Cassytha filiformis Emulgel in Murine Models OBJECTIVE: Develop an in vivo evaluation of a herbal emulgel, alone and in combination with the plant extracts of Wedelia chinensis and Cassytha filiformis, to promote hair growth..

[Audio] 1. Plant materials and preparation of the extract The plants Wedelia chinensis and Cassytha filiformis were collected, and A 50 grams portion of the powder was defatted with petroleum ether and subsequently macerated with hydroalcoholic (30:70) for 5 to 7 days Plant 1 Wedelia chinensis Plant 2 Cassytha filiformis.

[Audio] 2. Extraction ( C-O-L-D maceration) CF WC.

[Audio] 3. Preparation & Evaluation of Herbal Hair Emulgel Separately, Carbopol 940 was dissolved in distilled water, stirred continuously to form the gel bases, and the formulation's pH was adjusted to 6-6.5 using Triethanolamine. The oily and aqueous phases were then separately heated to 60 degrees celsius. Next, we combined the aqueous and oil phases and continuously mixed them until they reached room temperature. Finally, the Emulgel formulation (2%) was prepared by gently combining the emulsion with the gel Evaluation.

[Audio] 4. Hair growth activity in the murine model Swiss male albino rats aged 6 to 8 months and weighing 150 to 190 gm were used in studies on testosterone induced hair loss. Hydroalcoholic extracts of Wedelia chinensis and Cassytha filiformis were used to prepare an herbal emulgel, and murine models, including testosterone/stress induced rats, were used to assess hair growth promoting activity. The animals were divided into five groups of six rats each: negative control, positive control (Finasteride and Minoxidil), test group I (CF 2% emulgel), test group 2 (WC 2% emulgel), and test group 3 (CF plus WC 2% emulgel)..

[image]. [image]. [image]. [image]. [image]. Back with solid fill.

[Audio] 5. Herbal Emulgel application on testosterone induced and stress induced rats Biopsy and Hair length estimation.

[Audio] 6. Hair Growth Activity Evaluation Hair growth activity was assessed visually. Biochemical and biological parameters, as well as follicular density, were measured. Histopathological analysis further revealed an increased anagen/telogen ratio in test group 3 (CF plus WC 2%) (2.25 ± 0.6584), which was significantly higher than in the negative control group (0.25 ± 0.0156; p = ***P < 0.001). Also, it surpassed both the individual CF 2% (1.75 ± 0.5096, p = **P < 0.01) and WC 2% (1.4 ± 0.5584, p = **P < 0.01) groups. Statistical analyses were conducted in GraphPad Prism 3 using one way anova followed by Tukey's post hoc test; *p < 0.05 and ***p < 0.001 were considered significant..

Testosterone-induce Alopecia model 22 ll[lllhlLl.

[Audio] Conclusion In this study, the hydroalcoholic extracts of Wedelia chinensis and Cassytha filiformis, the herbal emulgel, maintained appropriate pH, acceptable viscosity, and stability at room temperature. Herbal hair emulgels prepared from these extracts showed that the CF plus WC (2%) combination had greater potential to promote hair growth. Overall, these findings suggest that the herbal extracts could be a preferable option for future formulations..