Marcadores

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Integrantes: Álvaro Beltrán Jeanissa Cárdenas Fernanda Raiman Carla Rivera Gabriela Rubilar Cristian Valdebenito

Revisión Bibliográfica

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Ayudante alumna: Alexandra Gotschlich Docente: Héctor Contreras Biología Celular y Molecular 2020, Medicina

Marcadores

predictores de metástasis en exosomas

plasmáticos de pacientes con CRPC

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Introducción

Figura 1: CEB. ¿SERAN LOS EXOSOMAS EL PROXIMO CABALLO GANADOR? [Internet]. Edu.es. [citado el 15 de marzo de 2021]. Disponible en: https://ceb.edu.es/seran-los-exosomas-el-proximo-caballo-ganador/

SERAN LOS EXOSOMAS EL PROXIMO CABALLO GANADOR?

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Biomarcador miRNA-34a

Figura 2: Diagrama de elaboración propia. Fuente: Corcoran C, Rani S, O'Driscoll L. miR‐34a is an intracellular and exosomal predictive biomarker for response to docetaxel with clinical relevance to prostate cancer progression. The Prostate, 2014-09, Vol.74 (13), p.1320-1334.

mRNA de BCL-2 t miRNA-34a BCL-2 m iRNA-34a miRNA-ua BCL-2 Salida de citocromo C Activaciön de caspasas 1 Activaci6n de nucleasas Fragmentaciön del DNA

Exosomas de líneas celulares resistentes a docetaxel presentan baja expresión de miRNA-34a

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Biomarcador miR-375

miR-375 Epithelial differentiation EMT Tumor cell Invasion

Figura 3: Diagrama extraído desde Selth L, Das R, Townley S, Coutinho I, Hanson A, Centenera M et al. A ZEB1-miR-375-YAP1 pathway regulates epithelial plasticity in prostate cancer. Oncogene. 2016;36(1):24-34.

Mayor expresiön de YAPI en lineas celulares de Disminuciön de YAPI Baja expresiön de miRNA-375 miRNA-375 de de EMT MET Suprsiön de tumor Aumento miRNA-375 ZEBI Disminuciön Vim y FN-1 Disminuci6n invasiön, migraciön y crecimiento

Figura 4: Diagrama de elaboración propia. Fuente: Selth L, Das R, Townley S, Coutinho I, Hanson A, Centenera M et al. A ZEB1-miR-375-YAP1 pathway regulates epithelial plasticity in prostate cancer. Oncogene. 2016;36(1):24-34.

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Biomarcador miR-1290

Sigue sin determinarse un papel directo de miR-1290 en el cáncer de próstata. Altos niveles de miR-1290 se relaciona con la peor sobrevida global de pacientes con CRPC.

Figura 5: Diagrama de elaboración propia. Fuente: Endo Y, Toyama T, Takahashi S, Yoshimoto N, Iwasa M, Asano T et al. miR-1290 and its potential targets are associated with characteristics of estrogen receptor α-positive breast cancer. Endocrine-Related Cancer. 2012;20(1):91-102.

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Biomarcador miR-423-3p

Figura 6: Diagrama de elaboración propia. Fuente: Guo T, Wang Y, Jim J, Mao X, Stankiewicz E, Scandura G te al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front Cell Dev Biol. 2021. 7;8;602493.

CRPC -...............................................................................> miRNA-423-3P en exosomas CP sin miRNA-423-3p en exosomas CP sin tratamiento miRNA-423-3p en exosomas cp con ADT miRNA-423-3P en exosomas

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Biomarcador AR-V7

Chromosome xR.FL Gene 5 t' anscript .V7 Gene ranscript -R protein AR.FL mRNA exon 3 t 3 UTR AR-VI mRNA Hinge region AR-VI -spec hc

Figura 7: Zhang T, Karsh L, Nissenblatt M, Canfield S. Androgen Receptor Splice Variant, AR-V7, as a Biomarker of Resistance to Androgen Axis-Targeted Therapies in Advanced Prostate Cancer. Clinical Genitourinary Cancer. 2019; 18 (1): 1-10

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Biomarcador Caveolin-1

Figura 8: Diagrama de elaboración propia. Fuente: Lin C, Yun EJ, Lo UG, Tai Y, Deng S, Hernandez E, et al. The paracrine induction of prostate cancer progression by caveolin-1. Cell Death and Disease. 2019;10:834.

Proliferación Supervivencia Metástasis Resistencia

IkBa NF-kB1 NF-kB1 IKKß OCT 4 KLF4 SOX2 y C-MYC

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Discusión

Falta de especificidad

Caveo[llnrl HfgedC Céncær de memo

Posible análisis junto a otros exámenes

Figura 9: imagen de elaboración propia.

Discusión :D.m4a

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Conclusión

Figura 10: imagen de elaboración propia.

Detección Predicción Especificidad

Aislado Icono De La Casa Con La Señal Verde Fotos, Retratos, Imágenes Y Fotografía De Archivo Libres De Derecho. Image 78862412.

Aislado Icono De La Casa Con La Señal Verde Fotos, Retratos, Imágenes Y Fotografía De Archivo Libres De Derecho. Image 78862412.

Vector símbolo de la Cruz Roja no vale | Vectores de dominio público

Utilidad de biomarcadores en exosomas plasmáticos

Conclusion c.m4a

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Referencias bibliográficas

(1) Corcoran C, Rani S, O'Driscoll L. miR‐34a is an intracellular and exosomal predictive biomarker for response to docetaxel with clinical relevance to prostate cancer progression. The Prostate, 2014-09, Vol.74 (13), p.1320-1334. (2) Sanaz N, Ali M, Pascal H, Behzad B, Elham S, William C, Behzad M. The role of miR‐34 in cancer drug resistance. Journal of cellular physiology, 2020-10, Vol.235 (10), p.6424-6440. Disponible en: https://onlinelibrary-wiley-com.uchile.idm.oclc.org/doi/10.1002/jcp.29640 (3) Huang X, Yuan T, Liang M, Du M, Xia S, Dittmar R et al. Exosomal miR-1290 and miR-375 as Prognostic Markers in Castration-resistant Prostate Cancer. European Urology. 2015;67(1):33-41. (4) Selth L, Das R, Townley S, Coutinho I, Hanson A, Centenera M et al. A ZEB1-miR-375-YAP1 pathway regulates epithelial plasticity in prostate cancer. Oncogene. 2016;36(1):24-34. (5) Guo T, Wang Y, Jia J, Mao X, Stankiewicz E, Scandura G, et al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front. Cell Dev. Biol. 2021; 8:602493.Guo T, Wang Y, Jia J, Mao X, Stankiewicz E, Scandura G, et al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front Cell Dev Biol. 2021. 7;8:602493. (6) Nimir M, Ma Y, Jeffreys S, Opperman T, Young F, Khan T, et al. Detection of AR-V7 in Liquid Biopsies of Castrate Resistant Prostate Cancer Patients: A Comparison of AR-V7 Analysis in Circulating Tumor Cells, Circulating Tumor RNA and Exosomes. Cells. 2019, 8, 688. (7) Lin C, Yun EJ, Lo UG, Tai Y, Deng S, Hernandez E, et al. The paracrine induction of prostate cancer progression by caveolin-1. Cell Death and Disease. 2019;10:834. (8) Pereira V. Tapia G. Bologna R. CAVEOLINA-1 implicaciones fisiológicas y patológicas. Odontoestomatología. Montevideo, Uruguay. 2017; 19 (30): 92-98.

(1) Corcoran C, Rani S, O'Driscoll L. miR‐34a is an intracellular and exosomal predictive biomarker for response to docetaxel with clinical relevance to prostate cancer progression. The Prostate, 2014-09, Vol.74 (13), p.1320-1334. (2) Sanaz N, Ali M, Pascal H, Behzad B, Elham S, William C, Behzad M. The role of miR‐34 in cancer drug resistance. Journal of cellular physiology, 2020-10, Vol.235 (10), p.6424-6440. Disponible en: https://onlinelibrary-wiley-com.uchile.idm.oclc.org/doi/10.1002/jcp.29640 (3) Huang X, Yuan T, Liang M, Du M, Xia S, Dittmar R et al. Exosomal miR-1290 and miR-375 as Prognostic Markers in Castration-resistant Prostate Cancer. European Urology. 2015;67(1):33-41. (4) Selth L, Das R, Townley S, Coutinho I, Hanson A, Centenera M et al. A ZEB1-miR-375-YAP1 pathway regulates epithelial plasticity in prostate cancer. Oncogene. 2016;36(1):24-34. (5) Guo T, Wang Y, Jia J, Mao X, Stankiewicz E, Scandura G, et al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front. Cell Dev. Biol. 2021; 8:602493.Guo T, Wang Y, Jia J, Mao X, Stankiewicz E, Scandura G, et al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front Cell Dev Biol. 2021. 7;8:602493. (6) Nimir M, Ma Y, Jeffreys S, Opperman T, Young F, Khan T, et al. Detection of AR-V7 in Liquid Biopsies of Castrate Resistant Prostate Cancer Patients: A Comparison of AR-V7 Analysis in Circulating Tumor Cells, Circulating Tumor RNA and Exosomes. Cells. 2019, 8, 688. (7) Lin C, Yun EJ, Lo UG, Tai Y, Deng S, Hernandez E, et al. The paracrine induction of prostate cancer progression by caveolin-1. Cell Death and Disease. 2019;10:834. (8) Pereira V. Tapia G. Bologna R. CAVEOLINA-1 implicaciones fisiológicas y patológicas. Odontoestomatología. Montevideo, Uruguay. 2017; 19 (30): 92-98.