Novel Electrospun mats for Tissue Engineering Applications

Novel Electrospun mats for T issue E ngineering Applications.

ELECTROSPINNING PROCESS. Fibers of nanometer range High surface area to volume ratio Extracellular matrix biomimetic structures High biocompatibility.

Synthesis of crosslinking agents. Electrospun gelatin nanofibers: A facile cross-linking approach using oxidized sucrose - ScienceDirect.

a) Non cross-linked mats dissolved completely with in 1 h.

INVITRO CYTOTOXICITY TEST - DIRECT CONTACT ASSAY WITH L929 Cells.

Improvement in quality of fabricated novel electrospun mats.

Conclusion and Future Perspective. Biopolymer based electrospun mats using biomolecule derived crosslinking agents could be fabricated in a single step First study being reported for the fabrication of albumin nano fibers using sucrose aldehyde and gum arabic aldehyde as crosslinking agents Cross-linked mats had improved properties compared to that of non cross-linked mats like lower swelling ratio, higher aqueous stability and higher thermal stability Biological studies of the fabricated electrospun mats showed cyto-compatibility Better biological properties when compared with GTA cross-linked mats Studies carried out in ground condition proved, the fabricated mat is a good scaffold material for growth of cells further studies on the novel fabricated mats can be extended under microgravity conditions to study the various cell responses in microgravity environment Fabricated scaffold can be used to culture cells and study the response of cells in various space missions like Gaganyan projects..

1) Jalaja , K., and Nirmala R. James. "Electrospun gelatin nanofibers: A facile cross-linking approach using oxidized sucrose." International journal of biological macromolecules 73 (2015): 270-278 . 2) Jalaja , K., et al. "Modified dextran cross-linked electrospun gelatin nanofibers for biomedical applications ." Carbohydrate polymers 114 (2014): 467-475. 3 ) Campiglio , Chiara Emma, et al. "Cross-linking strategies for electrospun gelatin scaffolds ." Materials 12.15 (2019): 2476 4 ) Hyo Won Kwak, Chemical and physical reinforcement of hydrophilic gelatin film with dialdehyde nanocellulose , International Journal of Biological Macromolecules, 146(2020):332-342..

DIRECTOR , VSSC GD, HRAG & HEAD, PQCD HEAD , DEPT OF CHEMISTRY, IIST ORGANISING COMMITTEE , QUALITY DAY -2021.