MRSA Nasal Carriage – Al-Reyada University

PREVALENCE OF NASAL CARRIAGE. METHICILLIN-RESISTANT.

[image]. CHAPTER 01. INTRODUCTION and BACKGROUND.

WHAT IS MRSA?. Methicillin-Resistant Staphylococcus aureus — a global antimicrobial resistance threat.

JUSTIFICATION. Why This Study. › METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS is resistant to commonly-used antibiotics, complicating treatment and increasing healthcare burden..

OBJECTIVES. General. Determine the prevalence of MRSA nasal carriage and associated risk factors among Al-Reyada University students..

[image]. CHAPTER 02. LITERATURE REVIEW. Historical background · Pathophysiology · Clinical features · Global prevalence.

HISTORICAL TIMELINE of MRSA. From first discovery to a global public health crisis.

PATHOPHYSIOLOGY and VIRULENCE of MRSA. Core Resistance Mechanism.

GLOBAL and REGIONAL MRSA PREVALENCE. 27.1%. Global (WHO 2025).

[image]. CHAPTER 03. MATERIALS and METHODS. Study design · Population · Sampling strategy · Lab protocols · Ethics.

STUDY DESIGN and POPULATION. Study Design. Study Type.

STUDY DESIGN and POPULATION. INCLUSION CRITERIA. ✓ Enrolled Al-Reyada undergrads ✓ Age ≥ 18 years; both genders ✓ Written informed consent ✓ No symptoms at sample collection.

STUDY DESIGN and POPULATION. Proportional Stratified Sample Distribution (n = 150).

STUDY DESIGN and POPULATION. Proportional Stratified Sample Distribution (n = 150).

LABORATORY METHODS. 1. Nasal Swab Collection. 2. Transport & Plating.

LABORATORY METHODS. 1. Nasal Swab Collection. Sterile cotton swab inserted 1–2 cm into each nostril, rotated for 5–10 sec. Placed in labeled sterile containers immediately..

LABORATORY METHODS. 2. Transport & Plating. Swabs processed within 4 hrs. Inoculated onto Mannitol Salt Agar (MSA) — selective for S. aureus. Incubated at 37°C for 18–24 hrs..

LABORATORY METHODS. 3. Identification. Gram staining confirms Gram-positive cocci. Catalase & coagulase tests confirm S. aureus. Colonies sub-cultured on Blood Agar..

LABORATORY METHODS. 4. MRSA Confirmation. Antibiotic susceptibility tested on Müeller-Hinton Agar with Cefoxitin discs (30 µg). Inhibition zone ≤ 21 mm = MRSA (CLSI criteria)..

[image]. CHAPTER 04. RESULTS. Prevalence findings · MRSA proportion · Risk factor associations.

KEY FINDINGS. Al-Reyada University Students, Aden, Yemen · n = 150.

RISK FACTOR ANALYSIS. MRSA Rate By Age 21-18 25-21 30-25 8.5 3.2 0.

RISK FACTOR ANALYSIS. 10 7 Isolates MRSA (58.8%) MSSA (41.2%).

[image]. CHAPTER 05. DISCUSSION. Contextualizing our findings against regional & global literature.

DISCUSSING OUR RESULTS. S. aureus Carriage (11.3%).

DISCUSSING OUR RESULTS. Smoking:. Our Study. Smokers: 18.8% vs Non-smokers: 5.2% (p=0.040).

DISCUSSING OUR RESULTS. Microbiological Culture Results and Distribution of Bacterial Isolates.

[image]. CHAPTER 06. CONCLUSIONS and RECOMMENDATIONS.

CONCLUSIONS. 1. S. aureus nasal carriage prevalence was 11.3% (17/150) — a moderate colonization rate among university students in Aden..

RECOMMENDATIONS. University Administration. › Implement regular MRSA screening for students in dormitories & shared housing.

logo. last. Thank You. For Your Attention. This study serves as a reminder that effective prevention begins long before infection occurs. Every informed action taken today contributes to a safer healthcare environment tomorrow….

last. ﴿ ٱلْحَمْدُ لِلَّهِ الَّذِي بِنِعْمَتِهِ تَتِمُّ الصَّالِحَاتُ، وَبِفَضْلِهِ تُبْلَغُ الْغَايَاتُ. ﴾ اللَّهُمَّ اجْعَلْ هَذَا الْعَمَلَ خَالِصًا لِوَجْهِكَ الْكَرِيمِ، نَافِعًا لِلْعِلْمِ وَالْوَطَنِ، وَبَارِكْ فِيهِ وَتَقَبَّلْهُ مِنَّا بِقَبُولٍ حَسَنٍ. ﴿ وَآخِرُ دَعْوَاهُمْ أَنِ ٱلْحَمْدُ لِلَّهِ رَبِّ ٱلْعَالَمِينَ. ﴾.