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[Audio] Linux Linux is an operating system like Windows or OS X of Mac. To analyze NGS data, you need to learn Linux because most bioinformatics tools are designed to run on Linux. There are different distributions of Linux and we are going to use Ubuntu. http://www.hobo-geek.com/2013/01/the-best-linux-distribution-2013.html.
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[Audio] Connecting to Linux You can connect to my Linux server from your personal computer or laptop. If you use Windows, google for MobaXterm, find and install the free portable edition. Note that you can always stop the video, make sure that you follow, then resume it..
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[Audio] Connecting to Linux Once installed, run MobaXterm, start a new SSH session, and enter the remote host address (i.e., 140.116.48.90; port 1112). You can also specify your username (i.e., your student ID). Note that the first letter of your ID is in capital. Besides, some contents in the figures of this slide may not fit the descriptions. In that case, stick to the descriptions..
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[Audio] Connecting to Linux server After setting the session, a new window will pop up, where the password (i.e., your account name) is requested. Note that when you key in your password, it does not show on the screen. Press enter after entering your password..
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[Audio] You should then see something as follows if your login is successful. The left window works like a file manager while the right one is the command line interface we will be using and is called shell..
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[Audio] If you use Mac, go to Application -> Utilities and open “Terminal”. Use `ssh -p 1112 [email protected]` (see below for example, ssh stands for “security shell”) for connecting to the Linux server. The steps are similar when you are in a Linux system. Again, the password does not show when you type..
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[Audio] You should now change your password using the command `passwd`, simply type it and press enter. Please come up with a new password containing at least 10 characters of alphabets and digits. You need to enter both the old and new passwords. Again, the password does not show when you type..