HISTOTECHNOLOGY

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HISTOTECHNOLOGY.

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INTRODUCTION. The word histotechnology is made up of two words, Histo and Technology. Histo is the short form of histology which is simply defined as, ‘‘The microscopic study of tissues is known as Histology’’. Technology means the application of scientific knowledge by using machinery and equipments for practical purposes..

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Histotechnology is based on a number of procedures and methods. These procedures are used for a variety of reasons; like the diagnosis of diseases either in the tissues of human, animals or plants to studying the different diseases or their types..

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Histotechnologist / Histotechnician. Histotechnicians and histotechnologists are the medical associated persons of any medical laboratory who perform different histological techniques resultant in the diagnoses of any diseases. They may also help the researchers to conduct research projects..

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They may also help the professionals to instruct in these scientific techniques. A histotechnologist / histotechnician will prepare very thin slices of human, animal or plant tissues for microscopic examination..

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Difference between Histology and Histotechnology.

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Difference between Histotechnician and Histotechnologist.

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Histotechnologists are also responsible to manage the situations occur unexpectedly in the laboratory such as the problems with equipment or identifying fundamental health issues that may cause unfamiliar test results. The responsibilities of both require quick and accurate output even in stressful situations..

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Major steps in histotechnology. There are following five major steps in histotechnology : Fixation Processing Embedding Sectioning Staining.

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Major equipments in histotechnology. There are following major equipment used in histotechnology laboratory: Microscopes Tissue processor Microtome Embedding station Incubator.

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Water bath Water Distillation plant Tissue staining station Slide dryer Vacuum oven.

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Slide storage cabinets Blocks storage cabinets Hot plate Camel hair brushes Glass slides Coverslips Slide transport files Log book.

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Major stains in histotechnology. Usually two types of stains are used in histotechnology Routine staining Special staining.

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In routine staining, haematoxylin and eosin staining is used to stain different parts of the tissue in different colors for differentiate to each other. while special staining procedures are used according to the needs of the defined disease or research work like connective tissues, fat, carbohydrates, microorganisms, pigments etc. require a different/special stain to pick a different color for differentiation from the other tissues present in the organ. All these type of stains require different reagents as well as different staining procedures..

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