Welcome to IN - COE Genomic Lab

Welcome to IN - COE Genomic Lab.

What are Botanical Ingredients?. Botanicals are the natural herbal ingredients used to help maintain a healthy lifestyle The natural compounds in plants like sugar, amino acids, fatty acids, and nucleic acids are the primary phenolic compounds The secondary phenolic compounds include flavonoids, hydroxycinnamic acids, hydroxybenzoic acids, and Stilbenes Botanical innovations opened the door for Herbalife to develop products that help people experience healthier life.

Introduction To Botanical Identification. The demand for quality botanicals continues to increase to invest in their health and wellness. Adulteration : Where inferior botanical ingredients might be sold and used to produce cheaper products. Herbalife Nutrition goes the extra mile to verify the natural ingredients in our products. The forensic biotechnology used by the quality assurance team includes conducting molecular diagnostic tests as well as extracting plant DNA..

Why Authentication?. As we all know almost all commercial products has barcode which will be specific to each product, same way every plant species contains specific DNA sequence information stored in them, which are species specific. At Herbalife ( IN – COE) along with chemical methods, we are going to adapt PCR method and Genomic tools to Identify the origin of plant-based RM, traceable up to species level in our Products..

What is DNA?. DNA is double helical structure composed of nucleotides. The two helix are joined by hydrogen bonds. The DNA is identical in all living organisms, made up of Adenine, Thymine, Cytosine and Guanine. The backbone of DNA is made of phosphate while the hydrogen bond joins two opposite strands..

Botanical Identification and authentication: Process.

Sample Preparation for DNA analysis. Botanical materials like Root, Bark, Seed, fruit tissues will be required and to be homogenized for the analysis. Homogenized Finished Goods will be taken as such for analysis..

Plant DNA extraction. Plant DNA extraction will be done as follows Breaking of cells to open and release the DNA. Separate DNA from protein and other cellular debris. Precipitate DNA with suitable alcohol. Purify the DNA and confirm the quality..

Quality Control of DNA. Spectrophotometry-based techniques helps to calculate the concentration of DNA from the value of absorbance. Fluorometers in association with Qubit will assist to get concentration of DNA from the fluorescence resulting from the binding of a reporter dye. Electrophoresis-based technique provides an on chip-electrophoresis tool for sizing, quantitation and quality control of nucleic acids..

PCR: Polymerase Chain Reaction. PCR is an incredibly powerful DNA analysis technique. We in IN - COE going to adapt this method to authenticate the plant materials at genetic level. PCR is a routine analytical tool for quickly analyzing the ideal plant materials used in our products. This enables us to identify any possible adulteration at genetic level..

DNA Sequencing. DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information which are specific. We at IN – COE will be establishing the data base for the plant species used in Herbalife products..

Methods of DNA Sequencing. Sanger sequencing, was developed by two time Nobel Laureate Frederick Sanger and his colleagues in 1977, hence the name the Sanger Sequence. In Sanger sequencing, the target DNA is copied many times, making fragments of different lengths. Fluorescent “chain terminator” nucleotides mark the ends of the fragments and allow the sequence to be determined. Next-generation sequencing techniques are new, large-scale approaches that increase the speed and reduce the cost of DNA sequencing...

Authentication of DNA Using BLAST Technique. BLAST (Basic Local Alignment Search Tool) refers to a suite of programme used to generate alignment between a nucleotides. A BLAST search enables a scientist to compare a subject nucleotide sequence (called a query) with a library or database of sequences, and identify database sequences that resemble the query sequence above a certain threshold, and provide BLAST score. BLAST score will assist scientist to authenticate the plant species. More the BLAST score, more is resemblance.

Genomic lab Instrumentation. The Invitrogen Qubit 4 Fluorometer is the fluorometer designed to accurately measure DNA, RNA, and protein quantity. Agilent Tape Station , is an automated electrophoresis tool for DNA and RNA sample quality control. Fully automated sample processing enables the unattended analysis of size, concentration and integrity. The plate centrifuge makes the safe and quick spin down of droplets and condensation very easy. Sealed plates are loaded vertically, and surface tension keeps samples in place so there is no danger of spilling samples..

Genomic lab Instrumentation. Thermal Cycler is a laboratory apparatus used to amplify segments of DNA using PCR. The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted. The Electrophoresis unit is used to separate nucleic acids and proteins based on their size and charge, gel electrophoresis instruments are used to run and compare DNA samples. GelDoc System is an automated systems capable of sequencing DNA or analyzing fragments. It is an 8-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process-controlled environments..

Genomic lab Instrumentation. DNA Sequencers The MiSeq instrument is one of the smallest benchtop sequencers that can perform onboard cluster generation, amplification, genomic DNA sequencing, and data analysis, including base calling, alignment and variant calling, in a single run. Genetic Analyzer (Sanger Sequencer) is an automated systems capable of sequencing DNA or analyzing fragments. It is an 8-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process-controlled environments.

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