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[Audio] nematode Nematodes Ostracods Nematodes Copepods Polychaetes nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode Eusyllis Afficher l'image d'origine nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode nematode Afficher l'image d'origine % M E I O F A U N A Rotifers Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Eusyllis Afficher l'image d'origine nematode nematode Afficher l'image d'origine nematode Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Flèche Courbe 1 mm 40 µm Macrofauna Turbellarians Gastrotrichs Afficher l'image d'origine C:\Users\ASUS\Desktop\Speach-7 Copie.png C:\Users\ASUS\Desktop\Speach-7 Copie.png Abundance: Max. 23 millions. m-2 Diversity: About 9000 described species.

[Audio] Collection Imag0016 Imag0014. [image] micro logiciel.

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[Audio] Afficher l'image d'origine Afficher l'image d'origine C:\Users\ASUS\Desktop\ghg.jpg Monotony and Redundancy in published studies Afficher l'image d'origine Afficher l'image d'origine Handicap 2: Slowness of responses in term of community diversity Handicap 1: Slowness of microscopic observations.

[Audio] Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Multispecies Approach Afficher l'image d'origine Monospecies Approach Afficher l'image d'origine species Mono Multi Sediment How ?.

[Audio] Mâle tête Copie Afficher l'image d'origine C:\Users\ASUS\Desktop\bizerte eau rouge-1-2-0-0--inkyfada.jpg Oncholaimus campylocercoides Natural Selection.

[Audio] Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Monospecies studies??? High number and biomass (♀ plus ♂ plus Juveniles) Community.

[Audio] Afficher l'image d'origine Desert Landscape Desert Landscape Desert Landscape Desert Landscape Desert Landscape Desert Landscape Eau de mer Sédiment azoique TRAITE Compartiment A Sédiment naturel (avec les nematodes) Compartiment B Tubes contenant de l’huile de lin (pour rendre l’air anoxique) Dispositif expérimental 2 Dispositif expérimental l.

[Audio] Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine How ?? Decrease OR increase in sediment grain size first and then monospecies studies??? Field L-A-B.

[Audio] Increase in sediment grain size Decrease in sediment grain size Mytilus galloprovincialis (shell) Posidonia oceanica (leaves) Squid Experimental design Selection phase : Azoic compartment with reduced grain size A compartment containing nematodes from the previous phase Progressive replacement of mineral with fine particles made with squid pens (10% by 10%) N = sédiment naturel; S / C = particules fines; CP = particules grossières; So = poudre de l'endosquelette de Sepia officinalis; n = nombre de répliquât.

Abundance Species number Species richness Shannon index Pielou•s Evenness 3 2 1 o 1 Indices (log (X+I)) Ut P 33 M 33 PM p 33 66 66 66 P 100 100 100 Traitements.

[Audio] Species Bank Terschellingia longicaudata Metoncholaimus pristiurus Ptycholaimellus ponticus Oncholainus campylocercoides Marylynnia puncticaudata.

[Audio] CCI20102014_0002 Copie C:\Users\user\Desktop\My papers\Article Marylynnia n.sp\CCI20102014_0002 Copie (5).jpg C:\Users\user\Desktop\My papers\Article Marylynnia n.sp\CCI20102014_0002 Copie (4).jpg C:\Users\user\Desktop\My papers\Article Marylynnia n.sp\CCI20102014_0002 Copie (3).jpg C:\Users\user\Desktop\My papers\Article Marylynnia n.sp\CCI20102014_0002 Copie (2).jpg M hopperi M johanseni M macrodentata M mulsa M musharafii M annae M bellula M choanolaimoides M complexa M dayi M oculissoma M preclara M punctata M puncticaudata n sp. M quadriseta M steckhoveni M wieseri M dubia M effilata M eratos M gerlachi M gracila M puncticaudata n sp..

[Audio] Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Mitochondrial Dna Barcoding.

[Audio] Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Afficher l'image d'origine Vouchering of nematodes Steps: DNA extraction: Polymerase Chain Reaction (P-C-R--): denaturation plus hybridation plus polymerisation Mixture of reagents to start P-C-R--: amplicon plus primers plus TaqPolymerase plus dye plus milliQ water 1) Incubabtion of tubes at 95 degrees celsius during 3 minutes, 2) Addition of 4 µl HCl, 10 µl Tris HCl (pH = 8) and 5 µl of 2% Trition X-100 to each tube, 3) Incubation at 95 degrees celsius during 3 minutes, Microcentrifugeuse MiniStar Silverline V-W-R 96-well Thermal Cycler Applied Biosystems Veriti™.

[Audio] T-A-E buffer plus EtBr 4 µl of P-C-R product Preparing gel of agarose 1% Gel of agarose 1% Quantity of amplified D-N-A using Nanodrop technique? UV Trans illuminator Amplified D-N-A observed by fluorescence Bands of Amplified D-N-A Cutting of bands of gel containing amplified D-N-A QIAquick P-C-R Purification Kit Gene Ruler 1kb D-N-A Ladder.