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. Molecular Biotechnology (BIOC 408) Dr. Rania Hassan Mohamed.

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Therapeutic and commercial use of Biotechnology.

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Techniques using Biotechnology: i.e. Microarray. The DNA microarray relies on cDNA fragments from a sample to hybridize with synthetic ssDNA sequences (specificA, C, G, T combinations). The synthetic fragments (oligonucleotides or oligos) are the probes. The cDNA fragments are the targets. Tat ts Probes.

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Techniques using Biotechnology: i.e. Microarray. DNA hybridization is when a single-stranded DNA molecule (ssDNA) reanneals with another ssDNA from another source. If the original ssDNA strand has sequences that are complementary to the introduced ssDNA strand, the two strands form a dsDNA hybrid molecule with one strand from each. Com lementar DNA bases d•DåA the rew v•.ONA to a ccrnpk•nentæy.

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Techniques using Biotechnology: i.e. Microarray. Each feature may contain hundreds or thousands of identical probes (oligos). Each array may contain tens of thousands of features. Each feature is looking for a specific gene sequence of nucleotide bases Thousands of specific genes can be identified simultaneously. What is the DNA sequence for the feature in the graphic?.

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Techniques using Biotechnology: i.e. Microarray. Cy3 (Omen) cys (Red) GONA.

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Techniques using Biotechnology: i.e. Microarray. ///iii/'i/ö//.

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Therapeutic use of Biotechnology : 1. Monoclonal Antibody.

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Therapeutic use of Biotechnology : 1. Monoclonal Antibody.

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Therapeutic use of Biotechnology : 1. Monoclonal Antibody.

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Therapeutic use of Biotechnology : 1. Monoclonal Antibody.

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Therapeutic use of Biotechnology : 1. Monoclonal Antibody.

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Therapeutic use of Biotechnology : 2 . CAR-T cells.

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Therapeutic use of Biotechnology : 2 . CAR-T cells.

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Therapeutic use of Biotechnology : 2 . CAR-T cells.

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Therapeutic use of Biotechnology : 2 . CAR-T cells.

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Therapeutic use of Biotechnology : More commercial products 1- Antibiotics.

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Therapeutic use of Biotechnology : More commercial products 1- Antibiotics.

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Therapeutic use of Biotechnology : More commercial products 1- Antibiotics.

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Therapeutic use of Biotechnology : More commercial products 2 - Enzymes.

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Thank you.