Name: Application Of Plant Tissue Culture In Agriculture Horticulture And Forestry
Uploaded: 2024-07-30
Duration: 3 min 55 s
Description: Application Of Plant Tissue Culture In Agriculture Horticulture And Forestry.

Application Of Plant Tissue Culture In Agriculture Horticulture And Forestry. 

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Application Of Plant Tissue Culture In Agriculture Horticulture And Forestry

By NIVEDHA.R (192322061) AND SUMITHRA.T.S(192320056)

INTRODUCTION TO PLANT TISSUE CULTURE:. Plant tissue culture is a collection of techniques used to grow plants under sterile conditions on a nutrient culture medium.. 

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Plant tissue culture is a collection of techniques used to grow plants under sterile conditions on a nutrient culture medium.

PROCESS OF PLANT TISSUE CULTURE
Parent plant
oot
Plants are kept for
hardening in green
house ,sold to a
nursery and then
potted up.
Selection Of explant from
mother plant
Inoculation
Light &warmth for sh
initiation
Callus induction
Shoots are transferred to
Shoot formation
rooting media(higher
auxin conc)

BENEFITS OF PLANT TISSUE CULTURE:. Disease-Free Plants: Produces plants free from pathogens. Mass Propagation: Rapid multiplication of plants in a short time. Genetic Modification: Facilitates the development of plants with desirable traits.. 

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Disease-Free Plants: Produces plants free from pathogens.
 Mass Propagation: Rapid multiplication of plants in a short time.
 Genetic Modification: Facilitates the development of plants with desirable traits.

OVERVIEW OF APPLICATIONS:. Agriculture Disease Resistance Drought and Salinity Tolerance Nutritional Enhancement Mass Propagation Germplasm Conservation Horticulture Ornamental Plants Commercial Floriculture Plant Breeding Forestry Reforestation and Afforestation Restoration of Degraded Lands Conservation of Endangered Species Improved Wood Quality. 

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Agriculture Disease Resistance Drought and Salinity Tolerance Nutritional Enhancement Mass Propagation Germplasm Conservation
 Horticulture Ornamental Plants Commercial Floriculture Plant Breeding
 Forestry Reforestation and Afforestation Restoration of Degraded Lands Conservation of Endangered Species Improved Wood Quality

Eucalypt propagation and germplasm preservation: (a) Eucalyptus grandis × E. urophylla cuttings in a commercial nursery; (b-i) Corymbia torelliana × C. citriodora: (b,c) shoots in tissue culture; (d) axillary bud outgrowth in shoot culture; (e) callogenesis at the base of a shoot; (f) multiple shoot production in organogenic culture; (g) a plantlet; i.e., a shoot with adventitious roots; (h) shoots in cool storage; and (i) alginate-encapsulated shoot tips and nodes; i.e., synthetic seeds. Photographs: (a) I. Wendling, (b,h) S.J. Trueman, and (c-g,i) C.D. Hung.

AGRICULTURE APPLICATIONS - DISEASE RESISTANCE:. Case Study 1: Disease-Resistant Banana Plants Background: Panama disease caused by Fusarium wilt devastated banana plantations. Solution: Tissue culture techniques used to develop and propagate disease-resistant banana varieties. Impact: Increased banana yields and reduced crop losses.. 

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AGRICULTURE APPLICATIONS - DISEASE RESISTANCE:

Case Study 1: Disease-Resistant Banana Plants Background: Panama disease caused by Fusarium wilt devastated banana plantations. Solution: Tissue culture techniques used to develop and propagate disease-resistant banana varieties. Impact: Increased banana yields and reduced crop losses.

AGRICULTURE APPLICATIONS - VIRUS-FREE PRODUCTION:. 

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AGRICULTURE APPLICATIONS - VIRUS-FREE PRODUCTION:

Case Study 2: Virus-Free Potato Production
 Background: Potatoes are susceptible to various viral diseases.
 Solution: Meristem culture used to produce virus-free potato plantlets.
 Impact: Enhanced potato production and quality.

HORTICULTURE APPLICATIONS - ORCHID PROPAGATION:. Case Study 3: Orchid Propagation Background: Orchids are highly valued ornamental plants with slow natural propagation. Solution: Tissue culture techniques such as seed culture and micropropagation used for mass production. Impact: Large-scale production of orchids, making them more accessible and affordable.. 

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HORTICULTURE APPLICATIONS - ORCHID PROPAGATION:

Case Study 3: Orchid Propagation
 Background: Orchids are highly valued ornamental plants with slow natural propagation.
 Solution: Tissue culture techniques such as seed culture and micropropagation used for mass production.
 Impact: Large-scale production of orchids, making them more accessible and affordable.

HORTICULTURE APPLICATIONS - NEW FLOWER VARIETIES:. 

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HORTICULTURE APPLICATIONS - NEW FLOWER VARIETIES:

Case Study 4: Development of New Flower Varieties
 Background: High demand for new and unique flower varieties.
 Solution: Tissue culture combined with genetic engineering to create new varieties with desirable traits such as color, fragrance, and resistance to diseases.
 Impact: Diversified flower market and increased commercial value.

FORESTRY APPLICATIONS – REFORESTATION:. Case Study 5: Reforestation with Fast-Growing Tree Species Background: Deforestation and the need for sustainable wood production. Solution: Tissue culture techniques used to propagate fast-growing and high-yielding tree species like eucalyptus and poplar . Impact: Enhanced reforestation efforts and sustainable timber production.. 

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Case Study 5: Reforestation with Fast-Growing Tree Species
 Background: Deforestation and the need for sustainable wood production.
 Solution: Tissue culture techniques used to propagate fast-growing and high-yielding tree species like eucalyptus and poplar . Impact: Enhanced reforestation efforts and sustainable timber production.

FORESTRY APPLICATIONS – CONSERVATION:. Case Study 6: Conservation of Endangered Tree Species Background: Many tree species are endangered due to habitat loss and over-exploitation. Solution: Tissue culture methods employed for the conservation and reintroduction of endangered tree species into their natural habitats. Impact: Preservation of biodiversity and restoration of ecosystems.. 

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Case Study 6: Conservation of Endangered Tree Species
 Background: Many tree species are endangered due to habitat loss and over-exploitation.
 Solution: Tissue culture methods employed for the conservation and reintroduction of endangered tree species into their natural habitats.
 Impact: Preservation of biodiversity and restoration of ecosystems.

TECHNOLOGICAL ADVANCES:. Automated Systems: Use of robotics and automation for large-scale tissue culture production. Cryopreservation: Long-term storage of plant tissues at ultra-low temperatures for germplasm conservation. Genetic Engineering: Integration of tissue culture with genetic modification techniques to develop plants with improved traits.. 

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Automated Systems: Use of robotics and automation for large-scale tissue culture production.
 Cryopreservation: Long-term storage of plant tissues at ultra-low temperatures for germplasm conservation.
 Genetic Engineering: Integration of tissue culture with genetic modification techniques to develop plants with improved traits.

Agrobacterium
tumefaciens
Restriction
site
T DNA
Ti plasmid
DNA containing gene
for desired trait
Recombinant
Ti plasmid
Plant cell
1. Insertion of gene
into plasmid using
restriction enzyme
and DNA ligase
2. Introduction
into plant cells
in culture
1—-1 Regeneration
of plant
T DNA carrying
new gene within
plant chromosome
Plant with new trait

CHALLENGES:. High initial costs and technical expertise required. Risk of somaclonal variation leading to genetic instability.. 

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High initial costs and technical expertise required.
 Risk of somaclonal variation leading to genetic instability.

CONCLUSION:. Summary: Plant tissue culture is a powerful tool with significant applications in agriculture, horticulture, and forestry. Impact: Real-life case studies demonstrate its potential to enhance crop production, conserve biodiversity, and support sustainable practices. Future Outlook: Continued innovation and adoption of tissue culture techniques will drive further advancements in plant sciences.. 

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Summary: Plant tissue culture is a powerful tool with significant applications in agriculture, horticulture, and forestry. Impact: Real-life case studies demonstrate its potential to enhance crop production, conserve biodiversity, and support sustainable practices. Future Outlook: Continued innovation and adoption of tissue culture techniques will drive further advancements in plant sciences.