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      Now we understand the importance of analysis of mercury, so let's check out the method.

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      Now we understand the importance of analysis of mercury, so let’s check out the method..

      Scene 2 (8s)

      A rapid spectrophotometric method for the determination of mercury in fish samples using diphenylthiocarbazone.

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      Procedure: A series of standard solution of a neutral aqueous solution containing 1–250 µg of mercury(II) in a 10-ml volumetric flask was mixed with 0.8 ml of 1.45×10−3 M of the diphenylthiocarbazone reagent solution followed by the addition 0.1 ml of 4.5 M sulphuric acid (0.45 M ). After 1 min, 5 ml of 1,4-dioxane was added and the mixture was diluted to the mark with de-ionized water. The absorbance was measured at 488 nm against a corresponding reagent blank. The mercury content in an unknown sample was determined using a concurrently prepared calibration graph..

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      10–20 g of fish sample was taken in a 100 ml micro- Kjeldahl flask with a B24 socket attached to a standard double surface reflux condenser. The sample was digested according to the method recommended by the Analytical Methods Committee. The digest was filtered (if necessary) and neutralized with dilute ammonia in the presence of 1–2 ml of 0.01% (w/v) EDTA solution. The solution was transferred quantitatively into a 25-ml volumetric flask and diluted up to the mark with de-ionized water. An aliquot (1–2 ml) of the final solution was pipetted into a 10-ml calibrated flask and mercury content was determined as described under procedure using tartrate as a masking agent . The results of biological analysis by spectrophotometric method were found to be in excellent agreement with those obtained by atomic absorption spectrophotometric method (cold vapour technique)..

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      According to the EPA, the highest allowable average mercury concentration in fish per serving when eating 1 serving per week = 0.46 µg/g..